THE STUDY OF VDR FOKI RS2228570 SNP IN AUTOIMMUNE THYROIDITIS.

Autoimmune thyroid disease (AITD) is a common organ-specific autoimmune disease. A strong influence of genetic and epigenetic modifications has been demonstrated to take part in the development and progression of autoimmune thyroid diseases. The linkage between the Vitamin D receptor (VDR) polymorphism and several autoimmune disorders, including the AITD. In this article, we aim to investigate the Frequency of VDR Fokl (rs2228570) genotypes (CC, CT, TT) and alleles (C,T) in autoimmune thyroiditis. The investigation of VDR Fokl (rs2228570) was conducted on 150 samples (control (75 healthy women) and diseased women (75 diseased with autoimmune thyroiditis)) patients from the Adjara (Georgia) Population. It also examined some clinical and laboratory characteristics of the study population. Autoimmune thyroiditis's disease was diagnosed by measuring blood antibodies, determining the level of thyroperoxidase, and conducting an ultrasound examination. Anti-TPO and TSH were studied using the ELISA method. The genomic DNA was extracted from the peripheral blood. The polymerase chain reaction was evaluated to examine the VDR Fokl rs2228570 SNP polymorphism. According to VDR Fokl (rs2228570) genotypes (CC; CT, TT) frequency, in the control group, the Frequency of CC-genotype is 48%, CT-heterozygous genotype is 29.33%, and TT-genotype is 22.67%; in the diseased population, the Frequency of CC-genotype is 57.33%, CT-genotype is 34.67%, and TT-genotype is 8%. According to VDR Fokl (rs2228570) alleles (C, T), the Frequency of the C-allele is high, and the Frequency of the T-allele is low in both populations. The Frequency of the CC and CT genotypes of VDR Fokl (rs2228570) is high in the population with autoimmune thyroiditis compared to the control group; the TT genotype is relatively low in the population suffering from autoimmune thyroiditis; According to VDR Fokl (rs2228570) alleles (C, T), the Frequency of C-allele is high both population.

EVALUATION OF SOME LABORATORY PARAMETERS IN PATIENTS WITH MORBID OBESITY AFTER BARIATRIC SURGERY.

Obesity is a global challenge of the 21st century, both in terms of morbidity and mortality worldwide. In this article, we studied the effect of surgical treatment on glycemic indicators within morbid obesity patients, therefore assessing the glucose and glycosylated hemoglobin in blood serum. Also, we evaluated the hormonal background associated with Obesity - by measuring the insulin level in patients. This study included 40 patients who underwent bariatric surgery in the period 2017-2021; the above laboratory parameters were assessed before and after surgery. The control group consisted of 40 patients with morbid Obesity planning bariatric surgery in the future. Thus, we investigated three groups: I - control group - 40 patients with morbid Obesity who planning bariatric surgery in the future, Group II - 40 patients with morbid Obesity immediately before bariatric surgery and group III - the same 40 patients as in the second group, but after bariatric surgery performed between 2017 and 2021. It should be noted that the glucose level is ⁓1.4 times higher in patients immediately before bariatric surgery (group II) compared to the control group (group I) and ⁓1.19 times lower in the postoperative period (group III) according to compared with the control group. The insulin level is ⁓1.4 times higher in patients immediately before bariatric surgery (group II) compared to the control group (p=0.0066) and ⁓2.5 times lower in the postoperative period (group III) compared to the control group (p=0.0001). According to the results, the insulin level after surgery decreases by ⁓3.7 times compared to the preoperative group (p=0.0001). The glycated hemoglobin level in bariatric patients is lower (slightly) than in the control group (⁓1.01 times), which is explained by the preoperative preparation of the patient at least several months before surgery. In postoperative patients, it decreased by ⁓1.06 times compared to preoperative patients. Thus, today, bariatric surgery is undoubtedly a very effective method of radical treatment of morbid Obesity, which we can consider to be one of the most essential parts of the holistic model of obesity treatment.